|
Pathfinder | 人员疏散模拟软件,紧急疏散逃生评估系统 培训 |
|
班级人数--热线:4008699035 手机:15921673576( 微信同号) |
增加互动环节,
保障培训效果,坚持小班授课,每个班级的人数限3到5人,超过限定人数,安排到下一期进行学习。 |
授课地点及时间 |
上课地点:【上海】:同济大学(沪西)/新城金郡商务楼(11号线白银路站) 【深圳分部】:电影大厦(地铁一号线大剧院站)/深圳大学成教院 【北京分部】:北京中山学院/福鑫大楼 【南京分部】:金港大厦(和燕路) 【武汉分部】:佳源大厦(高新二路) 【成都分部】:领馆区1号(中和大道) 【广州分部】:广粮大厦 【西安分部】:协同大厦 【沈阳分部】:沈阳理工大学/六宅臻品 【郑州分部】:郑州大学/锦华大厦 【石家庄分部】:河北科技大学/瑞景大厦
开班时间(连续班/晚班/周末班):2020年3月16日 |
课时 |
◆资深工程师授课
☆注重质量
☆边讲边练
☆若学员成绩达到合格及以上水平,将获得免费推荐工作的机会
★查看实验设备详情,请点击此处★ |
质量以及保障 |
☆
1、如有部分内容理解不透或消化不好,可免费在以后培训班中重听;
☆ 2、在课程结束之后,授课老师会留给学员手机和E-mail,免费提供半年的课程技术支持,以便保证培训后的继续消化;
☆3、合格的学员可享受免费推荐就业机会。
☆4、合格学员免费颁发相关工程师等资格证书,提升您的职业资质。 |
☆课程大纲☆ |
|
- QuantiScan是一款操作简单、功能专一的凝胶扫描分析软件。QuantiScan还可以帮你分析,扫描多种多样的材料成分。可以将普通的办公扫描仪转换为功能强大的显像密度计,用于分析凝胶,污点和薄层层析板等。
-
- QuantiScan for Windows has similar functionality to sophisticated densitometers but at a fraction of the cost. It is able to analyze a wide variety of material including polyacrylamide and agarose gels, autoradiograms, TLC plates, etc. The program will accept images for analysis directly from any TWAIN compliant scanner as well as most BMP, JPG or uncompressed TIF files. For laned gels of reasonable quality, the analysis is highly automated, allowing extremely high throughput of data. Almost any type of image can be analyzed with the manual modes of Quantiscan. Images can be pre-processed in a variety of ways within QuantiScan including crop, flip, invert, scroll and zoom.
-
-
- Numeric, graphical and image output can be printed as reports and can also be saved as files or copied to the clipboard. The X,Y coordinates of densitometric profiles can output into a tabbed table for re-plotting in presentation graphics software such as Biosoft's FigSys. There is a separate module for analysis of discrete blots which can also be operated with automatic or manual blot detection.
-
- Lane Analysis Mode
- There is sophisticated lane processing and analysis for gels. Lanes of any width can be created manually or by using the unique automatic lane detection mode. There is also a facility to automatically set lanes at regular intervals for use with plates that have a preconfigured regular lane array. Lanes can be skewed or curved to allow for gel imperfections. Lane boundaries can be copied and the copies moved precisely into position with the cursor keys, for exact comparisons. Each lane can be labelled with an unique number both on its image and its densitometric profile plot. There are six options for automatic background subtraction: Minimum, Interpolated minimum, Valley, Edge, Polynomial and Manual. User-selected areas of images can be displayed as 3-D density maps which can be printed, saved or copied to the clipboard.
-
- Once a lane has been defined, its densitometric profile plot is automatically drawn. Peaks can then be specified on the profile either manually by marking their center and edges, or automatically using a sophisticated peak search routine. Where appropriate, automatic fitting of Gaussian peaks can be performed. This automatic fitting uses a least-squares minimization technique to fit a sequence of peaks and a polynomial background to the given profile. The image of the currently-selected lane is shown laid-out horizontally under the lane densitometry profile. A cursor-controlled vertical line going across the profile and the lane image aids user examination of the peaks. The image and profile of a standard lane can be added for comparison with the first. Their alignment can be adjusted to aid accurate side-by-side comparisons. Peak areas are calculated by integration using Simpson's rule and, if the automatic fitting option has been used, the area of the fitted peak is also automatically calculated.
-
- Calibration to a lane containing standard proteins may be made for automatic calculation of molecular weights. Calibration can also be made with a known standard solution such as those supplied with assay kits (just enter the position of its start line, its mol. wt. factor and the position of the solvent front, to give automatic calculation of mol. wts of unknowns). Parameters of any standard lane can be saved for between-plate comparisons. Calibration curve graphs and their fitted coordinates can be exported. Reports are generated for all peak parameters, including position, height, width, background and net area of peaks and, together with the graphical results, these can be printed, saved to disk or copied to the clipboard for transfer to other programs.
-
- Blot Analysis Mode
- QuantiScan offers a separate mode to analyze discrete blots. The blots can be automatically detected or manually defined. Their number, position, height, area and densitometric volume are automatically reported. There is a special module for automatic analysis of regularly arrayed blots. Automatic background subtraction is an option. Calibration, based on densitometric volume of standard blots can be done and this allows blot mode to be used for assays.
|
|
|
|
|
|